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Plasticity of BioPhi-driven humanness optimization in ScFv-CD99 binding affinity validated through AlphaFold, HADDOCK, and MD simulations

BioPhi-guided humanization was utilized to enhance the humanness of a humanized single-chain variable fragment targeting CD99, leading to the development of two variants: HuScFvMT99/3BP and HuScFvMT99/3HY. The HuScFvMT99/3BP variant incorporated framework region modifications, leading to modest impr...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Kanokporn Sornsuwan, Thanathat Pamonsupornwichit, On-anong Juntit, Weeraya Thongkum, Nuchjira Takheaw, Kanchanok Kodchakorn, Chatchai Tayapiwatana
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2025-01-01
Schriftenreihe:Computational and Structural Biotechnology Journal
Schlagworte:
BioPhi
Humanized ScFv
Binding affinity
AlphaFold
HADDOCK
MD simulations
Online-Zugang:http://www.sciencedirect.com/science/article/pii/S2001037025000017
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