Comparison of library preparation protocols and bioinformatic pipelines in high-throughput 16S rRNA gene sequencing

Abstract Background 16S rRNA gene sequencing is widely used for bacterial community profiling in both clinical and research contexts. The expanding availability of library preparation protocols and bioinformatic pipelines increases analytical flexibility but may also introduce method-dependent biase...

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Detalhes bibliográficos

Principais autores:	Olivia Andersson, Anna Fagerström, Katharina Dannenberg, Jan Kekki, Julia Rode, Ignacio Rangel, Carl Mårten Lindqvist, Bianca Stenmark
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2026-07-01
coleção:	BMC Microbiology
Assuntos:	16S rRNA sequencing Microbial profiling Bioinformatics Library preparation Amplicon region Taxonomic resolution
Acesso em linha:	https://doi.org/10.1186/s12866-026-05344-6
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