Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content

Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are mediators of cell survival and pathogenesis by facilitating virulence factor dissemination and resistance to antimicrobials. Studies of OMV properties often focus on hypervesiculating Escherichia coli mutants that have increased O...

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Principais autores:	Shelby L. Reimer, Daniel R. Beniac, Shannon L. Hiebert, Timothy F. Booth, Patrick M. Chong, Garrett R. Westmacott, George G. Zhanel, Denice C. Bay
Formato:	Artigo
Idioma:	Inglês
Publicado:	Frontiers Media S.A. 2021-03-01
Series:	Frontiers in Microbiology
Assuntos:	outer membrane vesicles Escherichia coli ultracentrifugation ultradiafiltration hypervesiculation Tol-Pal system
Acceso en liña:	https://www.frontiersin.org/articles/10.3389/fmicb.2021.628801/full
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