Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology
Akaluc, an enzyme engineered from luciferase, provides a potential powerful tool for tracing transplanted cells in vivo because of its near-infrared emission light. To enable evaluation of its potency, we inserted the Akaluc gene at AAVS1 locus using CRISPR/Cas9 technology and generated a clonal hum...
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| Principais autores: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2021-10-01
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| Series: | Stem Cell Research |
| Acceso en liña: | http://www.sciencedirect.com/science/article/pii/S1873506121003792 |
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