Quantification of DNase type I ends, DNase type II ends, and modified bases using fluorescently labeled ddUTP, terminal deoxynucleotidyl transferase, and formamidopyrimidine-DNA glycosylase

Here we describe the substitution of fluorescently labeled ddUTP for dUTP in the TUNEL assay to allow quantification of generated fluorescence signals by epifluorescence microscopy. The capping of DNase type I 3′OH DNA ends using ddTUNEL was further combined with phosphatase treatment for detection...

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Detalles Bibliográficos

Autores principales:	David S. Baskin, Marsha A. Widmayer, Martyn A. Sharpe
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Taylor & Francis Group 2010-07-01
Colección:	BioTechniques
Materias:	TUNEL ddUTP formamidopyrimidine-DNA glycosylase DNase apoptosis mammary gland
Acceso en línea:	https://www.future-science.com/doi/10.2144/000113439
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