Widefield Two-Photon Excitation without Scanning: Live Cell Microscopy with High Time Resolution and Low Photo-Bleaching.

We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synaptically-driven Ca(2+) events in primary rat hippoc...

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Detalhes bibliográficos

Principais autores:	Rumelo Amor, Alison McDonald, Johanna Trägårdh, Gillian Robb, Louise Wilson, Nor Zaihana Abdul Rahman, John Dempster, William Bradshaw Amos, Trevor J Bushell, Gail McConnell
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Public Library of Science (PLoS) 2016-01-01
coleção:	PLoS ONE
Acesso em linha:	http://europepmc.org/articles/PMC4732674?pdf=render
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