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Phase-driven rewiring in Escherichia coli enhances coenzyme Q10 biosynthesis via temporal and energetic coordination

Abstract Coenzyme Q10 biosynthesis in Escherichia coli is constrained by kinetic mismatches between precursor synthesis and methylation, alongside bioenergetic uncoupling. We implemented an optogenetic phase-control strategy integrating dynamic light induction, ribosome binding site (RBS) engineerin...

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Principais autores: Hao Li, Yongjun Wu, Dongfang Qin, Jun Xie, Wei Huang, Ronghua Chen, Hongmei Liu, Ying Wang, Dandan Zhao
Format: Artigo
Sprog:Inglês
Udgivet: Springer 2025-11-01
Serier:Applied Microbiology and Biotechnology
Fag:
Coenzyme Q10
Metabolic engineering
Temporal regulation
Membrane potential
Multivariate modeling
Optogenetic
Online adgang:https://doi.org/10.1007/s00253-025-13619-7
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