Estimates of RelSeq, Mesh1, and SAHMex Hydrolysis of (p)ppGpp and (p)ppApp by Thin Layer Chromatography and NADP/NADH Coupled Assays

The Mesh1 class of hydrolases found in bacteria, metazoans and humans was discovered as able to cleave an intact pyrophosphate residue esterified on the 3′hydroxyl of (p)ppGpp in a Mn2+ dependent reaction. Here, thin layer chromatography (TLC) qualitative evidence is presented indicating the substra...

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Xehetasun bibliografikoak

Egile Nagusiak:	Katarzyna Potrykus, Nathan E. Thomas, Bożena Bruhn-Olszewska, Michał Sobala, Maciej Dylewski, Tamara James, Michael Cashel
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	Frontiers Media S.A. 2020-10-01
Saila:	Frontiers in Microbiology
Gaiak:	(p)ppGpp (p)ppApp RSH Mesh1 SAH stringent response
Sarrera elektronikoa:	https://www.frontiersin.org/articles/10.3389/fmicb.2020.581271/full
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