Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutations

Targeted mutagenesis of a promoter or gene is essential for attaining new functions in microbial and protein engineering efforts. In the burgeoning field of synthetic biology, heterologous genes are expressed in new host organisms. Similarly, natural or designed proteins are mutagenized at targeted...

Полное описание

Библиографические подробности

Главные авторы:	Herbert M. Huttanus, Ellin-Kristina H. Triola, Jeanette C. Velasquez-Guzman, Sang-Min Shin, Rommel S. Granja-Travez, Anmoldeep Singh, Taraka Dale, Ramesh K. Jha
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Frontiers Media S.A. 2023-07-01
Серии:	Frontiers in Bioengineering and Biotechnology
Предметы:	protein engineering synthetic biology promoter engineering mutagenesis polymerase chain reaction overlap extension PCR
Online-ссылка:	https://www.frontiersin.org/articles/10.3389/fbioe.2023.1202388/full
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