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Mutation detection using ligase chain reaction in passivated silicon-glass microchips and microchip capillary electrophoresis

The ligase chain reaction (LCR) following PCR is one of the most sensitive and specific methods for detecting mutations, especially single nucleotide polymorphisms (SNPs). Performing LCR in microchips remains a challenge because of the inhibitory effect of the internal surfaces of silicon-glass micr...

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Библиографические подробности
Главные авторы: Xing Jian Lou, Nicholas J. Panaro, Peter Wilding, Paolo Fortina, Larry J. Kricka
Формат: Artigo
Язык:Inglês
Опубликовано: Taylor & Francis Group 2004-09-01
Серии:BioTechniques
Online-ссылка:https://www.future-science.com/doi/10.2144/04373ST03
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