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Limitations and possibilities of low cell number ChIP-seq

<p>Abstract</p> <p>Background</p> <p>Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting material in the range of 1–20 million cells...

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Enregistré dans:
Auteurs principaux: Gilfillan Gregor D, Hughes Timothy, Sheng Ying, Hjorthaug Hanne S, Straub Tobias, Gervin Kristina, Harris Jennifer R, Undlien Dag E, Lyle Robert
Format: Artigo
Langue:Inglês
Publié: BMC 2012-11-01
Collection:BMC Genomics
Sujets:
PCR duplicates
Redundant reads
HTS
NGS
Next generation sequencing
Micro-ChIP
N-ChIP
Native ChIP
Location analysis
Histone
Accès en ligne:http://www.biomedcentral.com/1471-2164/13/645
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