An improved Xer-cise technology for the generation of multiple unmarked mutants in Mycobacteria
Xer-cise is a technique using antibiotic resistance cassettes flanked by dif sites allowing spontaneous and accurate excision from bacterial chromosomes with a high frequency through the action of the cellular recombinase XerCD. Here, we report a significant improvement of Xer-cise in Mycobacteria....
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Taylor & Francis Group
2020-02-01
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| Series: | BioTechniques |
| Assuntos: | |
| Acceso en liña: | https://www.future-science.com/doi/10.2144/btn-2019-0119 |
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