Single-embryo RNA sequencing for continuous and sex-specific gene expression analysis on Drosophila

Summary: Exploring early embryonic gene expression is challenging due to the rate of development and the limited material available. Here, we present a protocol for ordering Drosophila embryos along a developmental pseudo-time trajectory and determining the sex of the embryos using RNA-seq data. We...

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Bibliografische Detailangaben

Hauptverfasser:	J. Eduardo Pérez-Mojica, Lennart Enders, Kin H. Lau, Adelheid Lempradl
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2023-09-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Developmental Biology RNAseq Gene Expression
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166723005026
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