Homogeneous Noncompetitive Immunoassay Based on the Energy Transfer Between Fluorolabeled Antibody Variable Domains (Open Sandwich Fluoroimmunoassay)

The antigen-dependent stabilization of an anti-hen egg lysozyme (HEL) antibody HyHEL-10 variable region was monitored with fluorescence resonance energy transfer (FRET) between fluorolabeled heavy chain (VH) and light chain (VL) fragments. The VH and VL fragments labeled with succinimide esters of f...

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Detalhes bibliográficos

Principais autores:	H. Ueda, K. Kubota, Y. Wang, K. Tsumoto, W. Mahoney, I. Kumagai, T. Nagamune
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis Group 1999-10-01
Colecção:	BioTechniques
Acesso em linha:	https://www.future-science.com/doi/10.2144/99274st04
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