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Measuring the absolute abundance of the microbiome by adding yeast containing 16S rRNA gene from a hyperthermophile

Abstract High‐throughput sequencing (HTS) of 16S rRNA gene amplicons provides compositional information regarding the microbial community, but not the absolute abundance of the bacteria. We aimed to develop a standardized method for quantifying the absolute abundance of bacteria in microbiome studie...

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Principais autores: Ju Yeong Kim, Myung‐hee Yi, Myungjun Kim, Seogwon Lee, Hye Su Moon, Dongeun Yong, Tai‐Soon Yong
Format: Artigo
Sprog:Inglês
Udgivet: Wiley 2021-08-01
Serier:MicrobiologyOpen
Fag:
16S rRNA gene
absolute abundance
hyperthermophile
microbiome
Thermus aquaticus
Online adgang:https://doi.org/10.1002/mbo3.1220
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