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Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

Abstract The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added...

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Detalhes bibliográficos
Principais autores: Miao Zhang, Harriëtte Oldenhof, Bulat Sydykov, Judith Bigalk, Harald Sieme, Willem F. Wolkers
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Portfolio 2017-07-01
Colecção:Scientific Reports
Acesso em linha:https://doi.org/10.1038/s41598-017-06542-z
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