Highly efficient chromatin conformation capture with post-enrichment in single cells by HiChew

Abstract We present HiChew, a novel chromatin conformation capture method combining efficient sticky-end ligation with post-PCR methylation-based enrichment. HiChew achieves approximately 50% valid pair ratios compared to 8% for unenriched methods, while maintaining high sensitivity. Its single-cell...

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Autors principals:	Zhichao Chen, Yeming Xie, Chen Zhang, Chen Tan, Fengying Ruan, Wenfang Chen, Meng Luo, Mei Guo, Zhe Xie, Huimin Cao, Meifang Tang, Xiuqing Zhang, Ying Zhang, Chong Tang
Format:	Artigo
Idioma:	Inglês
Publicat:	BMC 2026-04-01
Col·lecció:	Genome Biology
Matèries:	Chromatin conformation capture Single-cell Hi-C HiChew Post-enrichment method DNA methylation High-throughput sequencing
Accés en línia:	https://doi.org/10.1186/s13059-026-04059-1
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