Alkyne lipids as substrates for click chemistry-based in vitro enzymatic assays

Click chemistry is evolving as a powerful tool in biological applications because it allows the sensitive and specific detection of compounds with alkyne or azido groups. Here we describe the use of alkyne lipids as substrates for in vitro enzymatic assays of lipid modifying enzymes. The small alkyn...

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Autors principals:	Anne Gaebler, Robin Milan, Leon Straub, Dominik Hoelper, Lars Kuerschner, Christoph Thiele
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2013-08-01
Col·lecció:	Journal of Lipid Research
Matèries:	ceramide synthase acyltransferase fatty acid amide hydrolase click lipid click-labeling lipid substrate
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S0022227520375441
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