Production of isotope-labeled nanobody KN035 in Pichia pastoris yields native-like conformation and PD-L1 binding activity comparable to KN035 expressed in mammalian cells
Stable isotope-labeled proteins are indispensable for NMR-based structural studies. E. coli is a cost-effective and high-yield expression host, but it often fails to support proper folding and post-translational modifications required for functional human proteins. While mammalian cell systems produ...
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| Hoofdauteurs: | , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
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KeAi Communications Co. Ltd.
2026-08-01
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| Reeks: | Magnetic Resonance Letters |
| Onderwerpen: | |
| Online toegang: | http://www.sciencedirect.com/science/article/pii/S277251622600001X |
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