Monitoring protein phosphorylation by acrylamide pendant Phos-Tag™ in various plants

The aim of the present study is to rationalize acrylamide pendant Phos-Tag™ in-gel discrimination of phosphorylated and non-phosphorylated plant protein species with standard immunoblot analysis, and optimize sample preparation, efficient electrophoretic separation and transfer. We tested variants o...

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Библиографические подробности

Главные авторы:	Slavka eBekesova, George eKomis, Pavel eKrenek, Petra eVyplelova, Miroslav eOvecka, Ivan eLuptovciak, Peter eIlles, Anna eKucharova, Jozef eSamaj
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Frontiers Media S.A. 2015-05-01
Серии:	Frontiers in Plant Science
Предметы:	Medicago sativa Arabidopsis thaliana Protein phosphorylation Hordeum vulgare mitogen activated protein kinase Triticum turgidum
Online-ссылка:	http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00336/full
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