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Engineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution

Abstract Background Mesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these proteases have an optimal catalytic temperature of approximately 50 °C, and t...

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Bibliografische gegevens
Hoofdauteurs: Hong-Yan Zhao, Hong Feng
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BMC 2018-06-01
Reeks:BMC Biotechnology
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Online toegang:http://link.springer.com/article/10.1186/s12896-018-0451-0
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