Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes

Peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins. So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties. He...

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Autors principals:	Rui-Rui Guo, Gerard Comamala, Huan-Huan Yang, Marius Gramlich, Ya-Min Du, Ting Wang, Anne Zeck, Kasper Dyrberg Rand, Li Liu, Josef Voglmeir
Format:	Artigo
Idioma:	Inglês
Publicat:	Frontiers Media S.A. 2020-07-01
Col·lecció:	Frontiers in Bioengineering and Biotechnology
Matèries:	acidic PNGase N-glycans glycoprotein deglycosylation Dyella japonica analytical glycoscience
Accés en línia:	https://www.frontiersin.org/article/10.3389/fbioe.2020.00741/full
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