A random fractional 13C labeling strategy for P. pastoris expressed eukaryotic membrane proteins for solid-state NMR studies

Solid-state NMR (SSNMR) has emerged as an important technique for characterizing membrane protein structures. Using sparse 13C labeling of membrane proteins can significantly enhance spectral resolution. In this study, we present an investigation of a random fractional 13C labeling approach for larg...

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Autors principals:	Meihui Sang, Zejun Fan, Qiongqiong Ren, Jing Liu, Shaokai Zhao, Shenlin Wang
Format:	Artigo
Idioma:	Inglês
Publicat:	KeAi Communications Co. Ltd. 2026-05-01
Col·lecció:	Magnetic Resonance Letters
Matèries:	Solid-state NMR Sparse 13C labeling Eukaryotic membrane proteins P. pastoris expression system
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2772516225000786
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