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A simple method for construction of <it>pir<sup>+ </sup></it>Enterobacterial hosts for maintenance of R6K replicon plasmids

<p>Abstract</p> <p>Background</p> <p>The R6K replicon is one of the best studied bacterial plasmid replicons. Replication of the R6K plasmid and derivatives harboring its γ origin of replication (<it>ori</it><sub>R6Kγ</sub>) is dependent on the <it>pir </it>gene-encoded π protein. Originally encoded...

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Autori principali: Kvitko Brian H, Bruckbauer Steven, Prucha John, McMillan Ian, Breland Erin J, Lehman Stephanie, Mladinich Katie, Choi Kyoung-Hee, Karkhoff-Schweizer RoxAnn, Schweizer Herbert P
Natura: Artigo
Lingua:Inglês
Pubblicazione: BMC 2012-03-01
Serie:BMC Research Notes
Accesso online:http://www.biomedcentral.com/1756-0500/5/157
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