Protocol to detect and quantify interactions between proteins expressed in Drosophila S2 cells

Summary: Here, we present a protocol to quantify interactions among difficult-to-express proteins from Drosophila cells using the select western blot-free tagged-protein interaction (SWFTI) assay. We describe steps for plasmid design, cell plating, protein expression, and immunoprecipitation prepara...

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Prif Awduron:	Cristina C. DeOliveira, Changfan Lin, Brian R. Crane
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Elsevier 2024-09-01
Cyfres:	STAR Protocols
Pynciau:	Molecular Biology Molecular/Chemical Probes Protein Biochemistry Protein expression and purification
Mynediad Ar-lein:	http://www.sciencedirect.com/science/article/pii/S2666166724003368
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