Development of a counterselectable seamless mutagenesis system in lactic acid bacteria

Abstract Background Lactic acid bacteria (LAB) are receiving more attention to act as cell factories for the production of high-value metabolites. However, the molecular tools for genetic modifying these strains are mainly vector-based double-crossover strategies, which are laborious and inefficient...

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Hlavní autoři:	Yongping Xin, Tingting Guo, Yingli Mu, Jian Kong
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	BMC 2017-07-01
Edice:	Microbial Cell Factories
Témata:	Lactic acid bacteria Temperature-sensitive plasmid Seamless mutagenesis Counterselectable marker pheS
On-line přístup:	http://link.springer.com/article/10.1186/s12934-017-0731-8
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