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Transcriptomic-based selection of reference genes for quantitative real-time PCR in an insect endosymbiotic model

Reference genes are a fundamental tool for analyses of gene expression by real-time quantitative PCR (qRT-PCR), in that they ensure the correct comparison between conditions, stages, or treatments. Because of this, selection of appropriate genes to use as references is crucial for proper application...

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Huvudupphov: Agnès Vallier, Elisa Dell’Aglio, Mariana Galvão Ferrarini, Ophélie Hurtado, Carole Vincent-Monégat, Abdelaziz Heddi, Rita Rebollo, Anna Zaidman-Rémy
Materialtyp: Artigo
Språk:Inglês
Utgiven: Frontiers Media S.A. 2023-04-01
Serie:Frontiers in Ecology and Evolution
Ämnen:
real-time PCR
insect
symbiosis
transcriptomic
normalization
Länkar:https://www.frontiersin.org/articles/10.3389/fevo.2023.1152183/full
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