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Construction of strong mammalian promoters by random cis-acting element elongation

Synthetic oligonucleotides containing one of four kinds of cis-acting elements, binding sites for activating protein-1 (AP-1), nuclear factor κB (NF-κB), CArG binding factor A (CBF-A), and nuclear factor Y (NF-Y), were randomly ligated to construct DNA fragments. These fragments were inserted into t...

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Hlavní autoři: Ryohei Ogawa, Go Kagiya, Tsutom Kodaki, Shigekazu Fukuda, Kazutaka Yamamoto
Médium: Artigo
Jazyk:Inglês
Vydáno: Taylor & Francis Group 2007-05-01
Edice:BioTechniques
On-line přístup:https://www.future-science.com/doi/10.2144/000112436
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