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A straightforward microfluidic-based approach toward optimizing transduction efficiency of HIV-1-derived lentiviral vectors in BCP-ALL cells

Background: HIV-1-derived lentiviral vectors (LVs) are capable of transducing human cells by integrating the transgene into the host genome. In order to do that, LVs should have enough time and space to interact with the surface of the target cells. Herein, we used a microfluidic system to facilitat...

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Main Authors: Seyed Esmaeil Ahmadi, Rima Manafi Shabestari, Amir Asri kojabad, Majid Safa
Format: Artigo
Language:Inglês
Published: Elsevier 2023-06-01
Series:Biotechnology Reports
Subjects:
Lentiviral vector
Microfluidics
Transduction efficiency
Acute lymphoblastic leukemia
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X23000127
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