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Endonuclease restriction-mediated real-time polymerase chain reaction: a novel technique for rapid, sensitive and quantitative detection of nucleic-acid sequence

The article reported a novel methodology for real-time PCR analysis of nucleic acids, termed endonuclease restriction-mediated real-time polymerase chain reaction (ET-PCR). Just like PCR, ET-PCR only required one pair of primers. A short sequence (Ss), which was recognized by restriction enzyme BstU...

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Principais autores: Yi Wang, Yan Wang, Lu Zhang, Machao Li, Lijuan Luo, Liu Dongxin, Hua Li, Xiaolong Cao, Shoukui Hu, Dong Jin, Jianguo Xu, Changyun Ye
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2016-07-01
Serier:Frontiers in Microbiology
Fag:
PCR
real time PCR
LOD
Nucleic acid detection
ET-PCR
Online adgang:http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01104/full
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