Western blotting of native proteins from agarose gels

We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provided native structures for a variety of proteins and...

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Bibliografske podrobnosti
Principais autores:	Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta
Format:	Artigo
Jezik:	Inglês
Izdano:	Taylor & Francis Group 2022-05-01
Serija:	BioTechniques
Teme:	agarose conformation specific monoclonal antibody GFP native gel electrophoresis PLXDC2 SARS-CoV-2 spike protein
Online dostop:	https://www.future-science.com/doi/10.2144/btn-2022-0012
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Western blotting of native proteins from agarose gels

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