Assessing Ploidy Level Analysis and Single Pollen Genotyping of Diploid and Euploid Citrus Genotypes by Fluorescence-Activated Cell Sorting and Whole-Genome Amplification

Flow cytometry is widely used to determine genome size and ploidy level in plants. This technique, when coupled with fluorescence-activated cell sorting (FACS), whole genome amplification and genotyping (WGA), opens up new opportunities for genetic studies of individualized nuclei. This strategy was...

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Principais autores:	Miguel Garavello, José Cuenca, Steven Dreissig, Jörg Fuchs, Andreas Houben, Pablo Aleza
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2019-09-01
coleção:	Frontiers in Plant Science
Assuntos:	flow cytometry triploid tetraploid fluorescence-activated cell sorting whole genome amplification SSR and SNP markers
Acesso em linha:	https://www.frontiersin.org/article/10.3389/fpls.2019.01174/full
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