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High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

Summary: Application of single-stranded DNA recombineering for genome editing of species other than enterobacteria is limited by the efficiency of the recombinase and the action of endogenous mismatch repair (MMR) systems. In this work we have set up a genetic system for entering multiple changes in...

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I tiakina i:
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Ngā kaituhi matua: Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2020-03-01
Rangatū:iScience
Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S2589004220301309
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