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Construction and modification of a low-copy plasmid-based infectious clone for GI-19 genotype IBV via Red/ET recombineering: A simplified and efficient reverse genetics system for coronavirus

Avian infectious bronchitis virus (IBV), a Gamma coronavirus (γ-CoV), is one of the most economically important pathogens affecting the global poultry industry. In this study, we established a novel reverse genetics platform of infectious clone of GI-19 genotype IBV. This platform integrates low-cop...

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Principais autores: Keyu Feng, Yajuan Li, Chaoyi Song, Guanming Shao, Hailong Wang, Jun Fu, Qingmei Xie
Formato: Artigo
Idioma:Inglês
Publicado: Elsevier 2026-07-01
Series:Poultry Science
Assuntos:
IBV
Coronavirus
Infectious clone
Red/ET recombineering
Reverse genetics
Acceso en liña:http://www.sciencedirect.com/science/article/pii/S0032579126005092
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