Protocol for stable isotopic tracing to assess cellular lipogenic activity in induced neural stem cells

Summary: Here, we present a protocol to assess the lipogenic phenotype of induced neural stem cells (iNSCs) using stable isotopic tracing. We describe steps for the culture and preparation of iNSCs, labeling with [13C6]-glucose and [13C5, 15N2]-glutamine, and the subsequent extraction of metabolites...

Fuld beskrivelse

Bibliografiske detaljer

Principais autores:	Rosana-Bristena Ionescu, Julie A. Reisz, Monika Dzieciatkowska, Daniel Stephenson, Alexandra M. Nicaise, Pranathi Prasad, Cory M. Willis, Marta Suarez Cubero, Luca Peruzzotti-Jametti, Frank Edenhofer, Christian Frezza, Stefano Pluchino, Angelo D’Alessandro
Format:	Artigo
Sprog:	Inglês
Udgivet:	Elsevier 2026-03-01
Serier:	STAR Protocols
Fag:	metabolism neuroscience proteomics stem cells protocols in metabolomics and lipidomics
Online adgang:	http://www.sciencedirect.com/science/article/pii/S2666166725007154
Tags:	Tilføj Tag Ingen Tags, Vær først til at tagge denne postø!