Rapid Selection against Truncation Mutants in Yeast Reverse Two-Hybrid Screens
The yeast reverse two-hybrid system is a powerful technique for isolating mutations in a protein that abolish its interaction with a known partner. Selection is based on abrogation of growth suppression imposed when wild-type interactions confer 5-fluoroorotic acid (5-FOA) sensitivity to yeast cells...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Taylor & Francis Group
2001-05-01
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| Schriftenreihe: | BioTechniques |
| Online-Zugang: | https://www.future-science.com/doi/10.2144/01305st03 |
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