连接蛋白Cx43参与冠状动脉平滑肌细胞的异质性促进冠脉再狭窄
目的研究猪冠状动脉平滑肌细胞异质性与连接蛋白Cx43关系,进一步阐明连接蛋白Cx43在冠状动脉再狭窄发生中的作用。方法分别培养正常及支架术后猪原代冠状动脉平滑肌细胞,正常组应用血小板源生长因子（PDGF-BB）进行诱导,两组细胞分别进行电子显微镜观察细胞形态及结构变化,采用Western Blotting方法及实时荧光定量PCR测定Cx43、Cx40、α-SMA、S100A4蛋白和mRNA表达;然后诱导组应用Cx43阻断剂进行干预,再次检测上述指标变化。结果正常冠状动脉平滑肌细胞可见:纺锤型（spindle-shaped,S-SMC）和长菱形（rhomboid,R-SMC）两类,以S-SMC为...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Chinês |
| Pubblicazione: |
Editorial Office of Chinese Journal of Laboratory Diagnosis
2014-01-01
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| Serie: | Zhongguo shiyan zhenduanxue |
| Soggetti: | |
| Accesso online: | http://ZSZD.publish.founderss.cn/thesisDetails?columnId=88485385&Fpath=home&index=0 |
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