Multi-marker comparative analysis of 18S, ITS1, and ITS2 primers for human gut mycobiome profiling

BackgroundGut fungi play crucial roles in human health. The profiling of the human gut mycobiome continues to progress. However, adjustments in the selection of ribosomal DNA marker regions can substantially affect the taxonomic resolution of a population. In particular, the impact of using primers’...

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Detalhes bibliográficos
Main Authors: Hiba Orsud, Sumaya Zoughbor, Fatima AlDhaheri, Khalid Hajissa, Manar Refaey, Suad Ajab, Khaled Alswaider, Nora Mohamed, Obaid Alkaabi, Zakeya Al Rasbi
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2025-11-01
Colecção:Frontiers in Bioinformatics
Assuntos:
gut mycobiome
ITS sequencing
18S rRNA
ALDEx2
multi-marker strategy
Acesso em linha:https://www.frontiersin.org/articles/10.3389/fbinf.2025.1690766/full
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