Optimized lentiviral vector transduction of adherent cells and analysis in sulforhodamine B proliferation and chromatin immunoprecipitation assays

Summary: Transduction with lentiviral vectors is a useful approach to study the molecular function of specific genes in mammalian cells. Here, we present a calcium phosphate-based transfection protocol that guarantees highly efficient production and delivery of lentiviral vectors in adherent culture...

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Bibliographic Details

Main Authors:	Luyuan Li, Jonathan C. Trent, Josiane E. Eid
Format:	Artigo
Language:	Inglês
Published:	Elsevier 2023-03-01
Series:	STAR Protocols
Subjects:	Cell Biology Cancer Molecular Biology Gene Expression Chromatin immunoprecipitation (ChIP) Protein Biochemistry
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723000679
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