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Fractionation of human serum lipoproteins by single-spin gradient ultracentrifugation: quantification of apolipoproteins B and A-I and lipid components

A sensitive and reproducible method has been developed for separation of the major serum lipoproteins from 1 ml or less of human serum by isopycnic density gradient ultracentrifugation. The serum, applied to a step gradient (total volume 12.8 ml), was spun for 48 hr at 38,000 rpm at 10°C and, in eac...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: J R Foreman, J B Karlin, C Edelstein, D J Juhn, A H Rubenstein, A M Scanu
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 1977-11-01
Schriftenreihe:Journal of Lipid Research
Schlagworte:
isopycnic density gradient ultracentrifugation
radioimmunoassay
abetalipoproteinemia
familial hypercholesterolemia
Online-Zugang:http://www.sciencedirect.com/science/article/pii/S0022227520415949
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