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Protocol for production of tonic CAR T cells with dasatinib

Summary: Chimeric antigen receptors (CARs) are synthetic molecules composed of an extracellular antigen-binding domain and an intracellular signaling domain, leading to tonic signaling and manufacturing challenges. We present a protocol for the expansion of tonic CARs by using a Food and Drug Admini...

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Principais autores: Léa Rosselle, Thibault Leray, Sandy Joaquina, Benjamin Caulier, Emmet McCormack, Pascal Gelebart, Sébastien Wälchli, Else Marit Inderberg
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2025-03-01
Serier:STAR Protocols
Fag:
cell culture
cancer
health sciences
immunology
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166724006944
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