A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screens

Abstract Mapping genetic interactions is essential for determining gene function and defining novel biological pathways. We report a simple to use CRISPR interference (CRISPRi) based platform, compatible with Fluorescence Activated Cell Sorting (FACS)-based reporter screens, to query epistatic relat...

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Bibliografske podrobnosti

Principais autores:	Alina Guna, Katharine R. Page, Joseph M. Replogle, Theodore K. Esantsi, Maxine L. Wang, Jonathan S. Weissman, Rebecca M. Voorhees
Format:	Artigo
Jezik:	Inglês
Izdano:	BMC 2023-10-01
Serija:	BMC Genomics
Teme:	CRISPR interference Genetic modifier Epistasis Genome-wide screen ER membrane protein complex Tail-anchored proteins
Online dostop:	https://doi.org/10.1186/s12864-023-09754-y
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