CRISPRi enables fast growth followed by stable aerobic pyruvate formation in Escherichia coli without auxotrophy
Abstract CRISPR interference (CRISPRi) was applied to enable the aerobic production of pyruvate in Escherichia coli MG1655 under glucose excess conditions by targeting the promoter regions of aceE or pdhR. Knockdown strains were cultivated in aerobic shaking flasks and the influence of inducer conce...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Maximum Academic Press
2022-02-01
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| Colección: | Engineering in Life Sciences |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1002/elsc.202100021 |
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