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Aromatic Amino Acid Mutagenesis at the Substrate Binding Pocket of Yarrowia lipolytica Lipase Lip2 Affects Its Activity and Thermostability

The lipase2 from Yarrowia lipolytica (YLLip2) is a yeast lipase exhibiting high homologous to filamentous fungal lipase family. Though its crystal structure has been resolved, its structure-function relationship has rarely been reported. By contrast, there are two amino acid residues (V94 and I100)...

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Principais autores: Guilong Wang, Zimin Liu, Li Xu, Yunjun Yan
格式: Artigo
语言:Inglês
出版: Wiley 2014-01-01
丛编:The Scientific World Journal
在线阅读:http://dx.doi.org/10.1155/2014/382581
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