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A protocol for single nucleus RNA-seq from frozen skeletal muscle

The authors detail a method to isolate nuclei for single nucleus RNA sequencing from frozen, biobanked human skeletal muscle samples. Single-cell technologies are a method of choice to obtain vast amounts of cell-specific transcriptional information under physiological and diseased states. Myogenic...

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Bibliographic Details
Main Authors: Tyler GB Soule, Carly S Pontifex, Nicole Rosin, Matthew M Joel, Sukyoung Lee, Minh Dang Nguyen, Sameer Chhibber, Gerald Pfeffer
Format: Artigo
Language:Inglês
Published: Life Science Alliance LLC 2023-05-01
Series:Life Science Alliance
Online Access:https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202201806
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