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Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

Many macrofungi are impractical or impossible to culture. Consequently, DNA for long-read sequencing required for the assembly of high-quality genomes must be isolated from samples taken from the environment. Collection is often in remote locations, limiting the options for stabilising samples to me...

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Tallennettuna:
Päätekijät: Leigh A. Burgoyne, Andy R. Nilsen, Teresa Lebel, Pamela S. Catcheside, Tom W. May, David Orlovich, Alan Kuo, Anna Lipzen, Kurt Labutti, Robert Riley, William Andreopoulos, Maxim Koriabine, Mi Yan, Vivian Ng, Igor V. Grigoriev, David E. A. Catcheside
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: MDPI AG 2025-06-01
Sarja:Journal of Fungi
Aiheet:
fungi
remote sampling
DNA extraction
chromatin
purification
long-read sequencing
Linkit:https://www.mdpi.com/2309-608X/11/7/490
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