Fast in vitro protocol for the visualization and quantitative high-throughput analysis of sprouting angiogenesis by confocal microscopy

Summary: We describe an optimized, cost-effective, reproducible, and robust protocol to study sprouting angiogenesis in glass-bottom 96-well plates by confocal microscopy, ideal for screening of drug or shRNA libraries. Effective and stable knockdown of gene expression in primary endothelial cells i...

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Bibliographic Details

Main Authors:	Lanette Kempers, Ivo van der Bijl, Anne-Marieke D. van Stalborch, Bas Ponsioen, Coert Margadant
Format:	Artigo
Language:	Inglês
Published:	Elsevier 2021-09-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell culture Cell-based Assays Developmental biology High Throughput Screening Microscopy
Online Access:	http://www.sciencedirect.com/science/article/pii/S266616672100397X
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