An improved method for extraction of microbial DNA from alkaline-saline soil

A modified method for the direct extraction of DNA from alkaline-saline soils with minimum DNA fragmentation and a possible reduction in chimera formation during polymerase chain reaction (PCR) was developed. The commercial extraction kit Power Soil DNA (Mo Bio™ Laboratories, Inc.) was used as a ref...

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Hlavní autoři:	Valentín Pérez-Hernández, Mario Hernández-Guzmán, César Valenzuela-Encinas, Rocío Alcántara-Hernández, Isabel Estrada-Alvarado, Luc Dendooven, Rodolfo Marsch, Federico Gutiérrez-Miceli, Víctor M. Ruíz-Valdiviezo, Joaquín A. Montes-Molina
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Sociedad Mexicana de la Ciencia del Suelo A. C. 2021-06-01
Edice:	Terra Latinoamericana
Témata:	dna extraction dna sequencing pcr amplification saline-alkaline soil
On-line přístup:	https://www.terralatinoamericana.org.mx/index.php/terra/article/view/887
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