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Mutational optimization of the coelenterazine-dependent luciferase from <it>Renilla</it>

<p>Abstract</p> <p><it>Renilla </it>luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one hundred molecules of coelente...

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Bibliografische gegevens
Hoofdauteurs: Woo Jongchan, von Arnim Albrecht G
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BMC 2008-09-01
Reeks:Plant Methods
Online toegang:http://www.plantmethods.com/content/4/1/23
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