Protocol for generating a human iPSC-derived tri-culture model to study interactions between neurons, astrocytes, and microglia

Summary: Human induced pluripotent stem cell (hiPSC) models enable disease modeling and drug screening, but standardized methods for multi-lineage co-culture remain limited. Here, we present a cryopreservation-compatible tri-culture system of neurons, astrocytes, and microglia. We describe steps for...

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Autors principals:	Alexandra M. Lish, Paige C. Galle, Gwendolyn A. Orme, Nancy Ashour, Sarah E. Heuer, Annabel J. Curle, Christina R. Muratore, Tracy L. Young-Pearse
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2025-12-01
Col·lecció:	STAR Protocols
Matèries:	cell Biology cell culture Immunology Neuroscience stem cells cell differentiation
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2666166725005581
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